Khushboo was born on 29 September 1970 as Nakhat Khan in Andheri (West), Mumbai. She had her primary education in Swami Muktananda High School located in Andheri area. She has three brothers. She is married to director Sundar C. Kushboo speaks Hindi, Tamil, Telugu, Marathi,and English fluently.
Khushboo started her career in the early 1980s as a child artist. Her first movie appearance was in The Burning Train 1980 in the song “Teri Hai Zameen Tera Aasman” followed by Thodisi Bewafaii (1980) in both films she had an uncredited role.
She had her first credited role in the 1981 film Laawaris, and Dard ka Rishta (1982). Sunil Dutt gave her large share screen appearance in Dard Ka Rishta. Between 1981-1985 as a child artist she did Aapas Ki Baat,Bemisal, Jaanoo, Patton Ki Bazi and Tan-Badan. Since then, she has acted in over 100 movies, some opposite leading actors such as Kamal Hassan, Rajnikanth, Vishnuvardhan, Venkatesh, Nagarjuna, Ravichandran, Mammootty, Mohanlal, Sathyaraj, and Prabhu. She played the role of the sister of Anil Kapoor in the 1985 hit Meri Jung, and danced with Javed Jaffrey in his first dancing role on screen in the superhit song “Bol Baby Bol, Mere Sang Dol”.She went to become the No1 South Indian leading actress during her period.Her career as Hindi film heroine was a disaster as both Deewana Mujhsa Nahi and Prem Daan were flops. Kushboo was introduced to South India through the Telugu movie Kaliyuga Pandavulu opposite Venkatesh, under the direction of K Raghavendra Rao. She is known for her TV show Jackpot and her serials Kalki, Janani and Kungumam. Apart from Tamil movies, she has also acted in various movies in Hindi, Kannada, Telugu, and Malayalam. In Kannada, She has acted in many movies with Ravichandran notably Ranadheera (1987), Anjada Gandu, Shanthi Kranthi and Yugapurusha.
Khushboo married actor-director Sundar C. They have two daughters, Avanthika and Ananditha, after whom they have named their production house. She has been living in Chennai for the past twenty years. She has three brothers who live in Chennai. She is one of the most popular and friendly stars. Kushboo learned classical dance from her co-star and friend, actress Sukanya, a student of Chennai Kalakshetra. Kushboo also mentioned in an interview to Vijay TV that Sukanya used to help with her hairstyles and dialogues for K. Balachander’s TV serials. One of her brothers, Abdullah, is expected to make his debut in the movie industry, playing the lead role in the movie Netru pol inru illai.
Khusboo made an announcement in April 2010 that she would be entering politics soon before the 2011 assembly elections and later joined the DMK, by signing her membership form at the party headquarters in Chennai.